漂白粉消毒后对虾养殖源水细菌群落的响应特征研究          下载免费PDF全文

王庚申  颜懿  李彤  董鹏生  谢建军  许文军  张德民  张化俊 《海洋与湖沼》2024,55(1):202-212

漂白粉消毒是凡纳滨对虾养殖源水常见的管控措施, 可有效控制病原菌传播; 然而,消毒会强烈扰动水体微生物群落,但源水细菌群落对漂白粉消毒的响应特征尚未阐明。在室内条件下, 设置高浓度(60 mg/L)和低浓度(20 mg/L)漂白粉消毒源水, 通过高通量测序及荧光定量PCR技术探究消毒后源水细菌群落、病原菌及抗生素抗性基因(ARGs)的动态响应规律。结果显示, 消毒后, 拟杆菌门(Bacteroidota)丰度上升, 高浓度组变形菌门(Proteobacteria)的丰度显著降低; 至第3天, 两组源水的细菌群落组成趋于一致。此外, 漂白粉消毒显著改变了源水的细菌群落结构及共现网络的复杂性。在控制水体病原菌方面, 消毒后病原菌的总丰度降低, 但种类增加, 高浓度漂白粉消毒对病原菌的抑制作用更加显著。漂白粉消毒对ARGs的去除具有选择性, 仅对sul1、floR、cfr、tetQ有一定的去除作用, 且高、低浓度对ARGs的去除无差异。综上, 60 mg/L可以作为漂白粉消毒养殖源水的更好浓度选择。研究结果从微生物生态视角评价了不同浓度漂白粉的消毒作用, 可为对虾养殖生产中的源水管控提供理论支撑。  相似文献   

Temporal and spatial distributions of dominant shrimp stocks and their relationship with the hydrological environment in the East China Sea   总被引：3，自引：0，他引：3  

吕华庆  宋海棠 《中国海洋湖沼学报》2007,25(4):386-397

To provide a scientific and technological base for fishery administration, holding a moratorium on fishing, and combating habitat degradation, a shrimp stock survey was carried out in May, August, and November 1998 and in February 1999. The study was conducted in the area between 26o00′ N and 33o00′ N and to the west of 127o00′ E in the East China Sea using a multi-sac trawl-net, with 115 stations being sampled. Up to 2001, we had found 121 species, which belong to 63 genera under 22 families, and 41 species are of high economic value and in great abundance. Nine shrimp species were of great economic importance, whose stock accounted for 76.8% of the demersal total. They were Parapenaeus fissuroides, Metapenaeopsis philippi, Palaemon gravieri, Metapenaeopsis barbata, Solenocera koelbeli, Solenocera crassicornis, Trachypenaeus curvirostris, Solenocera melantho and Parapenaeopsis hardwickii (listed in stock order). The nine species belong to the eurythermal and eurysaline community and high thermal and high saline community, had different migration patterns and stocks, and their distribution patterns could be generally classified into three types:(1) dominating in the north or the south;(2) dominating to the north of and in the coast to the south of 30o00′ N;and (3) dominating to the east of 60 m isobath, which were related to six water masses in the ECS near two lines, i.e., the 60 m isobath and 30o00′N latitudinal lines. Densely habited shrimps were found in all four seasons due to temperature and salinity frontiers and upwelling. The general stock density index was relatively higher in spring, summer, and autumn, when it surpassed 10 kg/h, while in winter, it was only 6.8 kg/h which might be caused by overfishing. According to the spawning areas of the nine species and their distribution patterns, to attain sustainable development of the shrimp fishery in the ECS, it is imperative to protect fishing areas and to hold a moratorium on catching to the west of 60 m isobath from April through October, and between 60 m and 100 m isobaths and to the south of 30oN from June through August and to limit fishing in winter.  相似文献   

磨刀溪梯级水电开发对河岸植物群落的影响   总被引：2，自引：0，他引：2       下载免费PDF全文

孙荣  袁兴中  刘红  黎璇 《水科学进展》2011,22(4):561-567

于2008年采用植被常规调查方法,对磨刀溪干流3个引水式电站对河岸植物的影响进行调查研究,按影响情况,各电站设3个采样点.用植物生活型、物种多样性等参数,衡量小水电对河岸植物群落的影响.共采集到维管植物157种,其中乔木21种,灌木19种,草本植物117种.结果表明:各样地之间河流生境物理因子(流速、水深、底质类型、底质异质性)之间存在显著性差异;总的物种丰富度和草本植物的物种丰富度在各样地间存在显著性差异,乔木和灌木物种丰富度差异不显著;物种多样性方面,各样地之间差异性显著;湿生植物物种丰富度分析表明,小水电的修建,造成坝下样地湿生植物成分较低,与坝上样地和电站出水口样地有明显差异.研究认为,引水式电站的修建使河流河岸植被的连续性受到干扰,不利于河流生物多样性的保护.  相似文献   

Analysis of Microbial Molecular Ecology Techniques in Constructed Rapid Infiltration System     

姜昕  马鸣超  李俊  鲁安怀  钟佐燊 《中国地质大学学报(英文版)》2011,(5)

The microbial molecular ecology techniques,which were developed on the basis of molecular,were applied in studying the bacteria in Constructed Rapid Infiltration(CRI) system.These techniques are very efficient in better describing the bacterial diversity,microbial community distribution,and relations between microbial group structure and nitrogen contamination through the analysis of microbial nucleic acid sequence fragment in CRI.The results further revealed the removal mechanism of contamination,which is ...  相似文献   

祁连山冻土区天然气水合物DK一2钻孔微生物群落     

韩路  武淑娇  李建华  吕杰  祝有海 《中国区域地质》2011,(12):1874-1882

对青海省祁连山永久冻土区天然气水合物DK一2钻孔的11件样品进行分析,通过微生物群落分析来探寻水合物层样品与非水合物层样品的差别。在11件样品中均发现了细菌16SrDNA,未检测到海洋天然气水合物地区常见的古菌16SrDNA、mc以（I,Ⅱ）、pmoA、mmoX和mxaF。分析得到的细菌16SrDNA分属5个门,包括变形杆菌门、放线菌门、拟杆菌门、厚壁菌门和异常球菌一栖热菌门,随着样品深度的增加,细菌多样性有降低的趋势。对非水合物层样品DK2—19和水合物层样品DK2—25进行细菌系统发育树分析,发现这2个样品群落结构相差较大。水合物层样品与非水合物层样品细菌群落对比后发现,水合物层样品中叫一变形杆菌的比例低于非水合物层样品中1一变形杆菌的比例,而Arthrobacter属多发现于非水合物层的样品中。  相似文献   

Biogeochemistry and carbon mass balance of a coccolithophore bloom in the northern Bay of Biscay (June 2006)     

J. Harlay  L. ChouC. De Bodt  N. Van OostendeJ. Piontek  K. SuykensA. Engel  K. SabbeS. Groom  B. DelilleA.V. Borges 《Deep Sea Research Part I: Oceanographic Research Papers》2011,58(2):111-127

Primary production (PP), calcification (CAL), bacterial production (BP) and dark community respiration (DCR) were measured along with a set of various biogeochemical variables, in early June 2006, at several stations at the shelf break of the northern Bay of Biscay. The cruise was carried out after the main spring diatom bloom that, based on the analysis of a time-series of remotely sensed chlorophyll-a (Chl-a), peaked in mid-April. Remotely sensed sea surface temperature (SST) indicated the occurrence of enhanced vertical mixing (due to internal tides) at the continental slope, while adjacent waters on the continental shelf were stratified, as confirmed by vertical profiles of temperature acquired during the cruise. The surface layer of the stratified water masses (on the continental shelf) was depleted of inorganic nutrients. Dissolved silicate (DSi) levels probably did not allow significant diatom development. We hypothesize that mixing at the continental slope allowed the injection of inorganic nutrients that triggered the blooming of mixed phytoplanktonic communities dominated by coccolithophores (Emiliania huxleyi) that were favoured with regards to diatoms due to the low DSi levels. Based on this conceptual frame, we used an indicator of vertical stratification to classify the different sampled stations, and to reconstruct the possible evolution of the bloom from the onset at the continental slope (triggered by vertical mixing) through its development as the water mass was advected on-shelf and stratified. We also established a carbon mass balance at each station by integrating in the photic layer PP, CAL and DCR. This allowed computation at each station of the contribution of PP, CAL and DCR to CO₂ fluxes in the photic layer, and how they changed from one station to another along the sequence of bloom development (as traced by the stratification indicator). This also showed a shift from net autotrophy to net heterotrophy as the water mass aged (stratified), and suggested the importance of extracellular production of carbon to sustain the bacterial demand in the photic and aphotic layers.  相似文献   

2009 年冬季南海北部网采浮游植物群落结构   总被引：1，自引：0，他引：1  

马威  孙军  田伟 《海洋科学》2011,35(9):8-13

根据2010年1月6～30日对南海北部的调查取样,作者对该海域网采浮游植物的分布和群落结构特征等进行了分析。共鉴定浮游植物4门52属155种（包括变形及变种）,主要以温带近岸和广布性种为主,其中硅藻有38属94种,占总种数的60．6％;甲藻有12属58种,占总种数的37．4％;浮游植物平均细胞丰度为36．46×10^4个／m^3,硅藻平均细胞丰度为13．77×10^4个／m^3,甲藻平均细胞丰度为1．39×10^4个／m^3,铁氏束毛藻（Trichodesmium thiebautii）平均细胞丰度为21．30×10^4个／m^3。粤东近岸区域是浮游植物高密集区,珠江口及其西南沿岸其次,而浮游植物稀疏区主要在调查区的中部海域,呈现出沿远岸区域逐渐降低的平面分布格局。除铁氏束毛藻为绝对优势种外,主要优势种有：菱形海线藻（Thalassionema nitzschioides）、短刺角毛藻（Chaetoceros messanensis）、圆筛藻（Coscinodiscus spp．）、粗根管藻（Rhizosolenia robusta）及角毛藻（Chaetoceros spp．）等。浮游植物群落香农．威纳物种多样性指数（H’）平均值为4．50,Peilou均匀度指数（J）平均值为0．81。浮游植物多样性程度较高,与细胞丰度平面分布基本呈相嵌分布,仅在近岸出现低值。  相似文献   

Ecology of macrobenthos in the south of Zhejiang coastal waters   总被引：1，自引：0，他引：1  

贾海波  胡颢琰  唐静亮  王益鸣  柴小平 《台湾海峡》2011,30(4):577-582

为了解浙江南部近岸海域海洋生态环境质量的现状及其变化趋势,2009年4月对浙江南部近岸海域的12个站位进行了大型底栖生物的分类、组成、丰度、生物量以及多样性的研究．应用Bray—Curtis相似性系数聚类和多维尺度排序,对浙江南部近岸海域的大型底栖生物进行群落划分;采用丰度生物量比较法分析群落的稳定性;并研究了对大型底栖生物产生影响的主要环境因子．本次调查共鉴定大型底栖生物109种,多毛类、软体动物和甲壳动物构成主要类群．平均生物量为23．38g／m^2,平均丰度为335．5ind／m^2,平均生物多样性指数为2．69．数量组成：生物量以软体动物居首（13．29g／m^2）,鱼类次之（3．68g／m^2）;丰度以多毛类居首（268．0ind／m^2）,软体动物次之（51．7ind／m^2）．浙江南部近岸海域可划分为3个群落．与环境因子进行相关性分析表明,有机污染含量、深度、溶解氧含量、盐度和悬浮物含量是对浙江南部近岸海域大型底栖生物产生影响的主要环境因子．总体上,除了瓯江口和乐清湾海域受到一定扰动,浙江南部近岸海域大型底栖生物环境质量较好,群落结构较为稳定．  相似文献   

2009年初夏长江口及毗邻海区表层浮游植物群落结构的色素表征   总被引：1，自引：0，他引：1  

余小青  金海燕  陈建芳  李宏亮  王奎  高生泉  卢勇  庄燕培 《海洋学研究》2011,(3):145-154

2009年6月对东海表层海水光合色素进行了高效液相色谱(HPLC)分析,通过藻类色素化学分类分析软件Chemical Taxonomy(CHEMTAX)获得了不同浮游植物对叶绿素a的贡献,研究了表层浮游植物群落结构的组成.结果表明:表层浮游植物的生物量与群落组成受温度、悬浮物质量浓度、营养盐等环境因素的影响,在水平分布...  相似文献   

渔业捕捞对威海港附近海域底上大型底栖群落结构影响的初步研究   总被引：1，自引：0，他引：1  

韩庆喜  李宝泉  韩秋影  张永  王跃启  王全超  刘东艳 《海洋通报》2011,30(2):121-126

根据2009年8月对威海港附近海域9个站位的拖网调查资料,初步分析了威海港近海底上大型底栖动物的群落结构特征,包括群落结构、种类组成、优势种、丰度和生物量、多样性等.应用PRIMIER 6.0进行了物种多样性、群落指数和ABc曲线等的分析.本次调查共采到底柄动物48种,其中甲壳动物19种,鱼类14种,各站位的大型底栖动...  相似文献